Shodhana (purification), one of the procedures mentioned in Ayurveda, has been recommended for many a drugs of both poisonous and non poisonous category, by different Ayurveda pharmacopoeias. For this purpose, various media, responsible for desired changes in the drug, are used before the use of certain poisonous plants in the formulation. UV spectroscopy technique was used to assess the changes taking place in the media, during shodhana procedure. In this study, the media, a mixture of takra (butter milk) and saindhava (rock salt) used, for seven days, for the shodhana of root of Langali (Gloriosa superba Linn.), a drug of upavisaha (semi-poisonous) group, was subjected to UV spectroscopic analysis. The result was analyzed by Principal Component Analysis (PCA) technique, using unscrambler software. Analysis of the results of UV spectroscopy from the range of 200 to 400 nm does not show significant changes, in the media, after four days. Physicochemical analysis of the samples before and after shodhana was also done. The values such as total ash, water and alcohol soluble extractive values of the shodhita samples were found to be increased. Qualitative analysis of samples show presence of alkaloids, saponin, tannins and carbohydrates before and after shodhana procedures.
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